At room Temperature:
4-6 hours at 66-78 degrees Fahrenheit

Cooler with frozen ICE packs:
24 hours at 59 degrees Fahrenheit

Refrigerator:
3-8 days at 39 degrees Fahrenheit or lower

Freezer:
6-12 Months 0-4 degrees Fahrenheit

Thawed Human milk:
Use within 24 hours

Source:  Medela 2009